Cryptosporidium is a common intestinal parasite that affects not only dogs, cats, cattle, but also human beings. It can cause symptoms like diarrhea, vomiting, gas, and abdominal discomfort in animals. The Canine Cryptosporidium Antigen Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Cryptosporidium antigen (Crypto Ag) in dog's feces or vomit specimen. Assay Time: 5-10 minutes
PRINCIPLE
The Canine Cryptosporidium Antigen Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is Cryptosporidium antigen in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Cryptosporidium antigen in the specimen.
REAGENTS AND MATERIALS
Test devices, with disposable droppers
Assay buffer
Cotton swabs
Products Manual
STORAGE AND STABILITY
The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date (24 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.
TEST PROCEDURE
Collect dog's fresh feces or vomit with the cotton swab from dog's anus or from the ground.
Insert the swab into the provided assay buffer tube. Agitates it to get efficient sample extraction.
Take out the test device from the foil pouch and place it horizontally.
Suck the treated sample extraction from the assay buffer
tube and place 4 drops into the sample hole "S" of the test device.
Note: If the liquid will not flow through the surface of the test strip in 30 seconds, please add another drop of the treated sample extraction.
Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
INTERPRETATION OF RESULTS
Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.
Negative (-): Only clear C line appear. No T line.
Invalid: No colored line appears in C zone. No matter if T line appears.
PRECAUTIONS
All reagents must be at room temperature before running the assay.
Do not remove test cassette from its pouch until immediately before use.
Do not use the test beyond its expiration date.
The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states.
LIMITATION
The Canine Cryptosporidium Antigen Rapid Test is for in vitro veterinary diagnosis use only. All result should be considered with other clinical information available with veterinarian. It is suggested to apply a further confirmative method such as PCR when positive result was observed.
J&G Biotech Ltd (Reg. No.: 08419172) 326 Cleveland Road, London, England E18 2AN, UK
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